Dietary Arachidonic Acid (20:4n-6) Levels and Its Effect on Growth Performance, Fatty Acid Profile, Gene Expression for Lipid Metabolism, and Health Status of Juvenile California Yellowtail (Seriola dorsalis)

Arachidonic acid (ARA, 20:4n-6) fed to Seriola dorsalis juveniles at different levels was evaluated. After a seven-week feeding trial, growth performance, hepatopancreas and muscle fatty (FA) composition, expression of lipid-relevant genes, blood parameters were Four isoproteic isolipidic experimental diets formulated contain 44% crude protein 11% lipids with graded inclusion ARA, 0% (Control), 0.4, 0.9, 1.4% the total diet. S. (14.54 ± 0.18 g) randomly divided into twelve tanks fifteen animals each. The hand three times per day apparent satiation. Dietary treatments did not significantly affect SGR, FCR, feed intake fish. Different ARA in directly influenced liver FA profiles, significant changes EPA deposition between Control treatment 1.4%, both tissues. arachidonate 5-lipoxygenase (alox5), acyl-CoA dehydrogenase very long chain (acadvl), carnitine O-palmitoyltransferase 1(cpt1a) affected by dietary treatments, an increasing accordingly levels. In contrast, reduction synthase (fas) proliferator-activated receptor alpha (ppara) reduced as increased addition, cortisol glucose found 0.9% level compared other treatments. Based on levels, gene for eicosanoids synthesis, lipid metabolic pathways, present study suggests maximum California yellowtail juveniles, dorsalis.